So far the literature consulted has not yielded any surveys demonstrating late effects with HVPC. As regards the non-maintenance of the superior performance in relation to group 1 from the 3rd week, if, on the one side, this may simply be a random occurrence, on the other side, it may be justified by the interruption of clearly the stimulation in the same week. Both aspects suggest that further surveys could explore the occurrence of these effects. On the other hand, it is necessary to also consider that HVPC has a negative late effect, as suggested by the significantly inferior performance of group 4, in the 4th and 6th weeks. Such findings have characteristics that are similar to those of the study by Baptista et al.24, using TENS (Transcutaneous Electrical Nerve Stimulation) in an attempt to accelerate sciatic nerve regeneration after crush injury.

The histological results of the two groups showed signs of impaired regeneration. Thus the negative results of group 4, in the 4th and 6th weeks, supported by the survey of Baptista et al.,24 suggest that this type of stimulation not only failed to improve the group’s performance, but also contributed to the negative effects in sciatic nerve regeneration. Finally, the group 5 was under the effect of simulated stimulation, therefore its performance was similar to that of the control group, as the animal did not suffer the effect of the applied current.

CONCLUSIONS HVPC proved efficient in the treatment of crush injury to nerves, when applied at any early stage on the area of the spinal cord and of the sciatic nerve root ganglion, with the dispersive electrode placed in the same contralateral region; HVPC demonstrated a late effect when applied at an early stage on the area of the spinal cord and of the sciatic nerve root ganglion, with the dispersive electrode placed on the gastrocnemius. Footnotes All the authors declare that there is no potential conflict of interest referring to this article. Study conducted in the Graduate Program in Orthopedics, Traumatology and Rehabilitation, Department of Health Sciences Applied to the Musculoskeletal System of the School of Medicine of Ribeir?o Preto of Universidade de S?o Paulo.
We often observe traumatic peripheral nerve lesions, especially in the nerves that pass through the limbs.

Injuries are usually caused by avulsion, compression, crushing, partial and total sectioning or stretching, resulting in the interruption of nerve impulses. This process can bring about loss or reduction of sensitivity and motor function in the innervated area, leading to countless nerve and Drug_discovery muscle abnormalities.1 Besides structural alterations in the muscle after nerve lesion, there are also metabolic alterations and gene expression of the musculoskeletal system such as the increase of acetylcholine receptors in the sarcolemma. Denervation causes an increase and proliferation of the extrajunctional acetylcholine receptors.