03, Fisher exact test). The Simple Shoulder Test score, the American Shoulder and Elbow Surgeons score, and all visual analog scale scores had improved significantly
(p < 0.01) at the time of the latest follow-up.
Conclusions: Total shoulder arthroplasty with a minimally PRN1371 cemented, all-polyethylene, pegged glenoid implant can yield stable and durable fixation at short to medium-term follow-up (mean, four years).”
“P>A group of selenium (Se)-hyperaccumulating species belonging to the genus Astragalus are known for their capacity to accumulate up to 0.6% of their foliar dry weight as Se, with most of this Se being in the form of Se-methylselenocysteine (MeSeCys). Here, we report the isolation and molecular characterization of the gene that encodes a putative selenocysteine methyltransferase (SMT) enzyme from the non-accumulator Astragalus drummondii and biochemically compare it with an authentic SMT enzyme from the Se-hyperaccumulator Astragalus bisulcatus, a related species that lives within the same native habitat. The non-accumulator enzyme (AdSMT) shows a high degree of homology with the accumulator enzyme (AbSMT) but lacks the selenocysteine methyltransferase activity in vitro, explaining why little or no detectable
levels of MeSeCys accumulation are AZD6094 Protein Tyrosine Kinase inhibitor observed in the non-accumulator plant. The insertion of mutations on the coding region of the non-accumulator AdSMT enzyme to better resemble enzymes that originate from Se accumulator species results in increased selenocysteine methyltransferase activity, but these mutations were not sufficient to fully gain the PLX3397 activity observed in the AbSMT accumulator enzyme. We demonstrate that SMT is localized predominantly within the chloroplast in Astragalus, the principal site of Se assimilation in plants. By using a site-directed mutagenesis approach, we show that an Ala to Thr amino acid mutation at the predicted active site of AbSMT results in a new enzymatic capacity to methylate homocysteine. The mutated AbSMT enzyme exhibited
a sixfold higher capacity to methylate selenocysteine, thereby establishing the evolutionary relationship of SMT and homocysteine methyltransferase enzymes in plants.”
“It has long been recognized that dyes other than disperse dyes would play a much larger industrial role if they could be applied to poly(ethylene terephthalate) (PET) fabrics at low temperatures. This research is related to a new process for the dyeing of hydrophobic PET with hydrophilic acid dyestuffs. The process is based on low-pressure plasma polymerization using an ammonia/acetylene gaseous mixture, which provides a nanoporous plasma coating containing accessible amine groups. Surface functionalization and crosslinking have been analyzed with X-ray photoelectron spectroscopy.