Although the precise nature of the mechanism(s) involved are unknown, there was no compelling evidence that this finding was related to the specific formulation of bupivacaine used (EXPAREL). The twofold lower AUC0–24h with EXPAREL add to favorites compared to bupivacaine solution reflects a slower absorption in dogs (2860 versus 6020ng·h/mL, P < 0.05 while in rabbits, the AUC0–24h values were not significantly different (1230 versus 1620ng·h/mL). Plasma concentrations of bupivacaine were approximately

similar or Inhibitors,research,lifescience,medical even more selleck products prolonged in rabbits (1.6 fold difference in AUC0–96hP < 0.05) consistent with sustained release of EXPAREL. As the toxicity of bupivacaine is known to be generally associated with its Cmax , the lower Cmax observed with EXPAREL as compared to bupivacaine solution at the same dose demonstrates potential safety advantages with this liposomal formulation. The rate of systemic

absorption of Inhibitors,research,lifescience,medical local anesthetics is dependent upon the total dose and concentration of drug administered, the route of administration, and the vascularity of the administration site. Absorption from the site of injection Inhibitors,research,lifescience,medical depends on the blood flow, the more rapid the rate at which plasma concentrations increase and the greater the peak concentration of the drug [7]. It is also possible that the interanimal variability in the PK response may be the consequence of unequal dispersion of the dosing material through the injection site resulting Inhibitors,research,lifescience,medical in packed material as well as drug-induced vasodilation so that varied amounts of drug were absorbed. In summary, a depot formulation with bupivacaine as the active component and DepoFoam lipid carrier was tested after PNB in rabbits and dogs. In the present studies, there were no local signs of toxicity, including no histological evidence for any increase in local reactions or general exacerbations

of bupivacaine toxicity after peripheral nerve block. Inhibitors,research,lifescience,medical Particularly, there was no evidence of nerve damage at doses up to 30mg/kg bupivacaine (more than threefold higher doses of EXPAREL versus bupivacaine solution). Bupivacaine did not impact directly on neural tissue, and the findings of granulomatous inflammation were more consistent with a nonspecific foreign—body type reaction most likely mediated by the DepoFoam particles. Importantly, the DepoFoam delivery system leads to a slower release of the drug allowing a longer Entinostat duration of action and, from a toxicological standpoint, to a slower uptake into the systemic circulation avoiding high plasma concentrations. 5. Conclusions In conclusion, a single administration of EXPAREL was demonstrated to be safe by peripheral nerve block in rabbits and dogs when tested in comparison with bupivacaine HCl and saline. EXPAREL did not cause overt irritation or local tissue damage even when injected at high dose or concentration around the brachial plexus nerve bundle.