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“Leucine-rich glioma inactivated 3 (LGI3) is known to be expressed mainly in the brain. However, the expression and physiological roles of LGI3 in skin cells remain unknown. In this study, it was found for the first time that LGI3 is expressed mostly by normal human keratinocytes. Furthermore, ELISA analysis showed that HaCaT human keratinocytes increased LGI3 secretion after exposure to ultraviolet B (UVB) in a time- and dose-dependent manner. We next GSK690693 chemical structure investigated the possible role of LGI3 in keratinocytes. LGI3 (50 ng/ml) increased survival of HaCaT cells by 20% after UVB irradiation
(150 mJ/cm2). It was also found that LGI3 stimulates the phosphorylation of Akt, which is involved in the cell survival-signalling cascade. Furthermore, LGI3 led to the phosphorylation of MDM2 and subsequent p53 degradation. Taken together, the data suggest
that LGI3 may regulate p53 levels and that keratinocyte-derived LGI3 may act as a novel cytokine for skin homoeostasis.”
“RNA-bmdmg proteins (RBP) can control gene expression at both transcriptional and post-transcriptional levels Plants respond to pathogen infection with rapid reprogramming of gene expression However, little is known Z-VAD-FMK concentration about how plant RBP function in plant immunity Here, we describe the involvement of an RBP, Arabidopsis thaliana RNA-bind mg protein-defense related 1 (AtRBP-DR1, At4g03110), in resistance to the pathogen Pseudomonas syringae pv tomato DC3000 AtRBP-DR1 loss of function mutants showed enhanced susceptibility this website to P syringae pv tomato DC3000 Overexpression of AtRBP-DR1 led to enhanced resistance to P syringae pv tomato DC3000 strains and dwarfism The hypersensitive response triggered by P syringae pv tomato DC3000 avrRpt2 was compromised in the Atrbp-dr1 mutant and enhanced in the AtRBP-DR1 overexpression line at early time points AtRBP-DR1 overexpression lines
showed higher mRNA levels of SID2 and PR1, which are salicylic acid (SA) inducible, as well as spontaneous cell death in mature leaves Consistent with these observations, the SA level was low in the Atrbp-dr1 mutant but high in the overexpression line The SA-related phenotype in the overexpression line was fully dependent on SID2 Thus, AtRBP-DR1 is a positive regulator of SA-mediated immunity, possibly acting on SA signaling-related genes at a post-transcriptional level”
“The present study was performed to survey the nature and extent of sequence conservation and divergence of miR165a and its target REVOLUTA (REV), a HD-ZIP III family member from Brassica encompassing both diploid and allotetraploid genomes. Post-transcriptional gene silencing (PTGS)-based regulation in plant requires precise pairing of the miRNA-mRNA target. We identified length and sequence polymorphisms in pre-miR165a, although 21 nt mature sequence was largely invariant.