(C) 2010 American Institute of Physics. [doi:10.1063/1.3488901]“
“Serving as a DNA
molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for C59 inhibitor the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by extraction with phenol/chloroform, precipitation with ethanol and mixing. Fragments of 100- to 1000-bp DNA were successfully amplified; the sequences showed 100% identity with lambda DNA. This prepared DNA marker displayed clear bands, indicating that it can CA3 datasheet be used for molecular studies.”
“The impact response of potassium bromide single crystals with < 100 > and < 110 > orientation was studied in a series of planar impact experiments under continuous monitoring of the sample rear surface velocity. The samples whose initial temperature varied between 166 and 880 K were loaded with aluminum, copper or tungsten impactors having velocities ranging from 140 to 690 m/s. The velocity histories recorded in
the experiments with maximum compressive stress, lower than about 2 GPa, include the elastic precursor wave followed by the plastic wave, while the waveforms, NSC23766 Cell Cycle inhibitor recorded in the experiments with stronger loading, are characterized by a three-wave structure caused by the shock-induced B1 -> B2 phase transformation
in KBr. On the basis of the recorded velocity histories, the temperature dependences of the shear stress tau(I) acting at the primary glide systems of KBr, of the transformation pressure p(tr), and of the characteristic transformation time t(tr) were determined. (C) 2010 American Institute of Physics. [doi:10.1063/1.3486015]“
“The development of meiotic division and associated genetic recombination paved the way for evolutionary changes. However, the secondary and tertiary structure and functional domains of many of the proteins involved in genetic recombination have not been studied in detail. We used the human Dmc1 gene product along with secondary and tertiary domain structures of Escherichia coli RecA protein to help determine the molecular structure and function of maize Dmc1, which is required for synaptonemal complex formation and cell cycle progression. The maize recombinase Dmc1 gene was cloned and characterized, using rice Dmc1 cDNA as an orthologue. The deduced amino acid sequence was used for elaborating its 3-D structure, and functional analysis was made with the CDD software, showing significant identity of the Dmc1 gene product in Zea mays with that of Homo sapiens.