Iridoid glycosides, secoiridoid glycosides and triterpene acids will be the essential constituents within the Fructus Ligustri Lucidi extract Survivin of FTZ, which include things like salidroside, oleuropeine aglycone, oleuropein, specnuezhenide, masilinic acid, pomolic acid acetate, oleanolic acid. Peak 13 showed a molecular ion at m/z 685 in MS spectra and exhibited m/z 523, 223 and 197 ions during the MS2 spectra. By comparison using the genuine standard, peak 13 was unambiguously identied as specnuezhenide. The identication of peak 19 as oleuropein was corroborated by detection of your molecular ion at m/z 539 and its aglycone fragment at m/z 377.

The MS spectrum showed a quasi molecular ion at m/z 539 plus the fragments ATP-competitive Chk inhibitor had been constant with all the following fragmentation pattern: the ion at m/z 377 arose from your reduction of glucose, the ion at m/z 307 was characteristic in the loss of a C4H6O fragment as well as fragment at m/z 275 may derive from the loss Eumycetoma of CH3OH from your elenolic fragment on the molecule. Peak 7 exhibited the pseudo molecular ion at m/z 377 in MS and characteristic ions at m/z 197 and m/z 153 in its MS2 spectrum, corresponding for the oleuropein aglycone or its isomer. By retrieving of literature information, peak 7 was identied as oleuropein aglycone. Among 51 analytes, there are 6 phenolic acids and three diterpenoids originated from Radix Salvia Miltiorrhiza. Phenolic acids could possibly be classied into monomer and polymer. Polymers can be composed of 1 or many unique monomers which include danshensu, caffeic acid or other individuals.

While in the MS2 spectra of three monomer standards, like small molecules such as CO2, CO and H2O had been created while in the fragmentation pathways, which AG-1478 ic50 indicated the presence of carboxyl, carbonyl or hydroxyl groups. Danshensu showed a ion at m/z 197, and created m/z 179 and m/z 135. Comparable to danshensu, the two in the ions at m/z 137 of protocatechuic aldehyde and m/z 153 of protocatechuic acid developed the identical ion at m/z 109 corresponding to your reduction of CO and CO2, respectively. As to three polymers, which contained an ester bond or ester bonds, the predominant fragmentation of their ions was the cleavage on the ester bond to lose danshensu and caffeic acid. As an illustration, peak ten exhibited a quasi molecular ion of m/z 717. Its MS2 spectra gave rise to prominent ion at m/z 519 corresponding to the loss of the molecule of danshensu. Other two fragment ions, ion at m/z 321 and ion at m/z 339 corresponding to the reduction of the 2nd danshensu as well as the rst caffeic acid. These information are consistent with individuals in the literature. Therefore, peak 10 was tentatively identied as salvianolic acid B. Similarly, peaks 9, 14 were identied as rosmarinic acid and salvianolic acid A individually.