On the other hand, it is easier to observe contamination of the other three reference strains by checking 5-FU mw the morphology on plate cultures. These results indicate the need for laboratories to review the processes of managing and preserving their control strains and working cultures, to avoid contamination during subculturing process. The phenotypic expression of the altered strains may not be apparent, and the impact of genetic drift may be silent (Ochman & Wilson, 1987) or affect properties such as toxinogenesis (Cryz et al., 1983; Ochman & Davalos, 2006). Moreover, the use of incorrect strains may have significant

impact for laboratories using molecular methods. Additionally, laboratories may wish to reconsider the benefits of single-use quality control materials, such as the LENTICULE discs that have been proven to exhibit no detectable changes (Desai et al., 2006). In summary, FAFLP has been proven as a valuable tool for assessing the genetic variability of isolates that have been preserved by various methods over a period of time. It also highlights the need for laboratories to validate their working cultures and stock cultures and consider using molecular methods. The authors would like to Metabolism inhibitor thank to Dr Barry Holmes for providing archived NCTC

cultures for this study. “
“The taxonomic status of a nitrogen-fixing bacterium, strain MSSRF38T, isolated from the rhizosphere of mangrove-associated wild rice Loperamide (Porteresia coarctata Tateoka), in Pichavaram, India, was studied using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the novel strain MSSRF38T was most closely related to Vibrio ruber DSM 16370T (98.3% gene sequence similarity), Vibrio rhizosphaerae DSM 18581T (98.2% sequence similarity) and <96% to the remaining Vibrio species. Multilocus sequence analysis using ftsZ, gapA, gyrB and mreB genes showed low levels

of gene sequence similarities (82–90%) with all species of the genus Vibrio with validly published names, indicating that strain MSSRF38T occupies a distinct phylogenetic position. DNA–DNA hybridization experiments showed that strain MSSRF38T had <70% DNA–DNA similarity to its closest neighbours V. ruber DSM 16370T (27.4%) and V. rhizosphaerae DSM 18581T (12.1%). Strain MSSRF38T could be differentiated from its relatives on the basis of several phenotypic characteristics. The major fatty acids were feature 3 (including C16:1ω7c and/or C15:0 iso 2-OH), C16:0, C18:1ω7c, C14:0 and C12:0. The DNA G+C content was 45.4 mol%. Based on genotypic, phenotypic, chemotaxonomic and DNA–DNA analyses, the name Vibrio mangrovi sp. nov. (type strain MSSRF38T=LMG 24290T=DSM 19641T) is proposed for this novel taxon.