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designed the study and SHN, SHL, YJL carried out western blotting. DWK, MYP and DJJ carried out immunohistochemistry and JHL, MKC drafted the manuscript. J-KP, CHK, SGK participated in the manuscript drafting and performed the statistical analysis. All authors read and approved the final manuscript.”
“Background The current treatment of hepatocellular carcinoma, especially hepatocellular carcinoma in middle and advanced stages, is a comprehensive therapy using a combination of surgery and chemotherapy.
Chemotherapy plays a critical role in the treatment of hepatocellular carcinoma. Nevertheless, multi-drug 3-deazaneplanocin A resistance (MDR) [1, 2] of hepatocellular carcinoma cells to multiple chemotherapeutics renders chemotherapy for hepatoma insufficient. Therefore, the target of drug resistance and its reverse strategy is one of the hotspots of mafosfamide hepatocellular carcinoma research. Establishing a reliable tumor MDR model is the foundation for the study of tumor MDR and its reversal. In this study, we established three different human hepatocellular carcinoma drug-resistance cell sub-lines of Bel-7402/ADM by applying ADM by three normal methods. We compared the biological characteristics the three cell sub-lines to acquire a comparatively ideal drug-resistance model which paved the way for revealing the clinical multidrug resistance phenomenon and the screening of a reversal agent. Materials and methods Cells and Animals Human hepatocellular carcinoma cell line Bel-7402 was purchased from Shanghai Institute of Biological Products.