Osteocytes secrete sclerostin along their dendrites in the canali

Osteocytes secrete sclerostin along their dendrites in the canaliculi after the cells become embedded in mineralized matrix [70]. Consistent with the high bone mass phenotype of sclerosteosis and van Buchem disease patients, mice with a deletion of Sost had dramatically increased bone mineral density that was due to increased bone Obeticholic Acid clinical trial formation rather than to decreased osteoclast activity [71] and [72], while overexpression of Sost decreased bone mass and strength due to decreased

bone formation [50]. Since the Wnt signaling pathway has been shown to be crucial in bone development, it has received much interest as a potential target for osteoporosis therapy [73]. Specifically, the genetic linkage of the high bone mass diseases sclerosteosis and van Buchem Lapatinib nmr disease to the SOST gene plus the specificity of sclerostin

in osteocytes point to sclerostin’s potential use as an anabolic bone agent. The only currently available anabolic drug for treating osteoporosis is teriparatide (Forteo®; Eli Lilly and Company, Indianapolis, IN) [74]. Teriparatide is the human recombinant form of parathyroid hormone (PTH) and acts through the PTH receptor. Patients receiving intermittent teriparatide treatment had higher bone mineral density than those treated with bisphosphonates [75]. Treatment with PTH drives bone formation by decreasing sclerostin expression  [76]. In wild-type and estrogen-deprived rats, PTH treatment directly regulated Sost transcription, decreased Sost/sclerostin expression, and increased bone mineral density [77]. When the PTH receptor was constitutively activated in osteocytes, selleck screening library mice had reduced sclerostin and increased bone mass. After the deletion of Lrp5 in these mice, the high bone mass phenotype was no longer apparent [78]. An alternative, but not mutually exclusive

model, is that PTH signals directly through LRP6 to activate β-catenin. Taken together, PTH functions as an anabolic bone agent through the osteocytes to decrease sclerostin expression and activate the Wnt/β-catenin pathway through Lrp5. Sclerostin antibodies are being developed to target the protein directly in order to improve bone mineral density. In preclinical studies, the administration of the sclerostin antibody AMG 785 (Amgen Inc., Thousand Oaks, CA) increased the formation of trabecular, periosteal, endosteal, and intractorical bone of postmenopausal osteoporotic rats [79] and cynomolgus monkeys [80]. In a phase I study in humans, a single dose of the sclerostin antibody increased bone mineral density in the hip and spine after 85 days relative to placebo controls [81]. In a phase II trial on postmenopausal osteoporotic women with femoral neck T-scores of − 3.

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