The selection medium was replaced every 3–4 days, the clones

that stably expressing GRP78-shRNAs were picked, expanded, cultured in the medium containing 200 μg/ml of G418, and identified by western blot and RT-PCR. RNA extraction and RT-PCR analysis Total RNA was isolated using Trizol (Invitrogen) according to the manufacture’s recommendation. 2 μg of total RNA from each samples were reverse transcribed using oligo(dT) primers at 37°C for 90 min. The relative mRNA levels were evaluated by quantitative PCR using SYBR green PCR kit (Takara). The signals were normalized to 18 S as internal control. The primers were as follows: MMP-2 Forward, 5’-ATAACCTGGATGCCGTCGT-3’ Reverse, 5’- AGGCACCCTTGAAGAAGTAGC-3’ MMP-9

Forward, 5’-GACAGGCAGCTGGCAGAG-3’ Reverse,5’-CAGGGACAGTTGCTTCTGG-3’ MMP-14 Forward,5’-CTGTCAGGAATGCTC-3’ Reverse, 5’-AGGGGTCACTTGAATGCTC-3’ TIMP-2 Forward, 5’-GAAGAGCCTGAACCACAGGT-3’ find more Protein Tyrosine Kinase inhibitor Reverse, 5’-CGGGGAGGAGATGTAAGCAC-3’ 18 S Forward, 5’-TCAAGAACGAAAGTCGGAGG-3’ Reverse, 5’-GGACATCTAAGGGCATCACA-3’ Western blot-analysis Cells were washed, harvested, lysed by lysis buffer (150 mM NaCl, 1% NP-40, 1% SDS, 1 mM PMSF, 10ug/ml Leupeptin, 1 mM Aprotinin,50 mM Tris-Cl, pH 7.4) on ice for 30 min and centrifuged at 12,000 g at 4°C for 10 min. The supernatants were quantified for protein concentration by BCA assay. Equal amounts of protein were loaded (50 μg per Regorafenib in vivo lane) and separated by 10% SDS-PAGE, transferred to PVDF membrane. The membrane was blocked with 5% non-fat milk for 2 h, incubated with a specific antibody (1:1000 dilution) for 3 h, stained with Geneticin mouse appropriate secondary antibody conjugated with HRP (1:2000 dilution) for 30 min at room temperature. After final washes, the membrane was developed using ECL reagent (Pierce, France). The levels of target proteins were normalized to β-Actin. Transwell invasion and wound healing assays Cells were harvested and seeded onto the fibronectin-coated, porous upper chamber inserts (105 per well) and allowed

to invade for 48 h. After 48 h, the inserts were inverted and stained with Hochest33258. Three fields were randomly chosen and the numbers of invaded cells were counted. The invasion potentiality of the GRP78 knockdown cells was measured by the average value of penetrated cells in three fields. For wound healing assay, the monolayer was carefully wounded by sterile pipette and washed with PBS for three times to remove the debris. The wounded monolayer was cultured in DMEM containing 1% BSA for 24 h, and photographed by microscope (×100). The status of wound closure was evaluated by inverted microscope. Cell proliferation assay Cells were seeded in 96-well culture plate at a density of 5 × 104/ml, 100 μl each well. The status of cell viability were monitored every 24 h. Briefly, the cells were washed with PBS for 3 times, 100 μl sterilized MTT solution (0.