The UCSF Committee on Animal Research permitted all procedures and researchers were qualified under the Animal Welfare Assurance Program. We used Alzet 2000 constant flow rate pumps to manage the cannabinoid receptor agonists systemically over a period of time of 2 weeks. Mice were split into four experimental groups. 0 or the pumps were filled with 50% DMSO/water as control. 013 M concentrations of WIN55,212 C2, ACEA or AM1241 dissolved in 50-yard DMSO/water. The pumps were placed in the back of each and every animal 4 days after tumor inoculation, when gross tumor formation was apparent. Under general anesthesia with isoflurane, a tiny incision was produced in the skin of the back. The pump was put subcutaneously and the incision was closed using surgical clips. Behavioral testing for physical allodynia was performed as described previously. Testing was performed by an observer blinded to the experimental groups, in the evening between 16:00 C19:00 h. Rats were put into a plastic cage with a wire mesh floor, allowing access to the feet. The tumor showing foot was examined using a digital von Frey anesthesiometer after half an hour was allowed for acclimatization. A positive response was observed if the foot was deliberately withdrawn and if there was a sudden inching upon application of a growing power with the von Frey firm probe tip. The withdrawal ceiling was as the pressure that was sufficient to elicit the above withdrawal response de Lymphatic system ned. The physical stimuli were presented at least 3 minutes apart allowing resolution of previous stimuli. Each animal was tested five times and the measurements were averaged and compared to the standard measurements for each animal which was obtained 24 hours and on the afternoon of inoculation just before tumor inoculation. Tumor growth was measured employing a 520M Plethysmometer to determine the paw amount. The pet s tumor bearing paw was introduced into a water mobile, which measures the change in pressure on account of engagement. Paw volume measurements were repeated 3 times and the results were averaged. The proportions for tumefaction development PFT �� and paw withdrawal were recorded on days 4, 7, 9, 11, 13, 15, and 18 days post inoculation. Data are reported as mean SE. Statistical analysis was done using posthoc and ANOVA Tukey s test. Immunohistochemistry of HSC3 cells implies that human oral cancer cells develop CBr1 and CBr2 in variety as shown from the homogeneous cytoplasmic immunoreactivity. The outcome of the western blot confirms the expression of CBr1 and CBr2 on two human oral cancer cell lines and shows that the cancer cell lines have an increased amount of CBr expression when compared with human NOKs. The best dose that showed an important decrease in stability on day 4 with each agonist was 2. 5 M.