Their high level of expression at the blood-brain, maternal-fetal, and

blood-testis barriers as well as their purported roles in oral absorption suggests that ABC transporters play important pharmacologic roles. Methods: We have developed a method to characterize the function and inhibition of ABC transporters using an automated cell counter with fluorescence detection capability. The assay was performed using stably-transfected HEK293 cells expressing P-gp, MRP1, or ABCG2 and examining transport of fluorescent substrates in the presence or absence of known inhibitors and compared to results obtained PHA-848125 with a flow cytometer. Fold increase in intracellular fluorescence was then calculated for cells incubated with fluorescent substrate in the absence of inhibitor versus in the presence of inhibitor. Results: Fold increase values obtained either with the cell counter or flow cytometer were comparable for cells expressing either MRP1 or ABCG2; slightly higher fold increase values were observed when cells expressing P-gp were read on a flow cytometer compared to the cell counter.

Discussion: The assay described provides an inexpensive detection method to aid in the development of Mocetinostat inhibitor novel ABC transporter inhibitors or to characterize potential drug-drug interactions. Published by Elsevier Inc.”
“The first aim of this study was to translate the Neck Pain and Disability Scale (NPAD) from English into Dutch producing the NPAD-Dutch Language ACY-738 cell line Version (DLV). The second aim was to analyze test-retest reliability and agreement of the NPAD-DLV and the Neck Disability Index (NDI)-DLV. The NPAD was translated according to established guidelines. Thirty-four patients (mean age 37.5 years, 68% female) with chronic neck pain (CNP), within an outpatient rehabilitation

setting, participated in this study. The NPAD-DLV and the NDI-DLV were filled out twice with a mean test-retest interval of 18 days. The intraclass correlation coefficient of the NPAD-DLV was 0.76 (95% confidence interval (CI) 0.57-0.87) and of the NDI-DLV 0.84 (95% CI 0.69-0.92). The limits of agreement of the NPAD-DLV and the NDI-DLV were, respectively, +/- 20.9 (scale 0-100) and +/- 6.5 (scale 0-50). The reliability of the NPAD-DLV and the NDI-DLV was acceptable for patients with CNP. The variation (‘instability’) in the NPAD-DLV total scores was relatively large and larger than the variation of the NDI-DLV.”
“Background: The impact of the Papanicolaou (Pap) smear on the prevention of cervical cancer is one of the greatest public health success stories. However, it is not clear if women understand the purpose of the Pap smear despite recent advancements and national attention over cervical cancer prevention.