Therefore, this study was carried out in order to evaluate substance use and sexual risk behaviour in a large German sample of HIV-positive MSM receiving specialized medical treatment. Results will be an empirical basis for the development of prevention strategies working with MSM diagnosed with HIV infection (‘prevention with positives’). Data were collected between January 2009 and February 2010. Participants were recruited in two specialized HIV out-patient clinics at university hospitals

in Germany. The interviewers, the attending physicians or nurses asked CH5424802 chemical structure patients if they wished to participate in a survey on sexual behaviour and substance use. Any interested patient received an information sheet on the study’s aims and content and on privacy. Participants signed an informed consent form; attendance was voluntary and without payment. Only HIV-positive MSM, who had known of their HIV-seropositive status for at least 12 months, were included. Exclusion criteria were insufficient German language ability and/or an acute psychotic disorder. A psychologist and trained medical students conducted the interviews. The ethics committee of the Medical Faculty at the University Duisburg-Essen, Essen, Germany, approved the study. Alcohol and illicit drug use Tanespimycin in vitro were examined using the German version of the semi-standardized interview European Addiction Severity Index

(Europ-ASI) [38]. Questions regarding sexual behaviour were based on the German KABaSTI Study of the Robert Koch Institute [39]. In addition, questions were asked regarding substance use in the immediate context of sexual behaviour: patients were asked buy Metformin whether they themselves or their sexual partners had consumed illicit drugs or alcohol until drunkenness immediately

before or during sexual intercourse in the last 12 months. First, we hypothesized that current substance use is associated with unprotected sexual intercourse. We differentiated between any unprotected sexual intercourse (including oral sex, which is less relevant for HIV transmission) and insertive and receptive anal sex. Secondly, we hypothesized that substance use in the immediate context of sexual activity is associated with unprotected sexual contacts. For the statistical analyses, spss® 17.0 (SPSS, Chicago, IL) was used. For group comparisons, the χ2 test or Fisher’s exact test was applied. If they fulfilled the criteria of normality and homoscedasticity, means were compared using a t-test for independent samples. In cases where these preconditions were not met, the nonparametric Mann–Whitney U-test was applied. In order to allow statements to be made regarding the 12-month prevalence of sexual risk behaviour, the analysis is solely based on those participants who had been sexually active during the 12-month period prior to the interview.