The mcnR

gene has one difference PD-0332991 research buy from the previously reported mdbA gene (O’Brien & Mahanty, 1994), which produces a frameshift in translation of the last 43 amino acids, alsoshortening the protein in 27 amino acids. The mcnI gene is identical to the previously reported MtfI immunity gene (93 amino acids). The polypeptide encoded possesses 43% identity and 72% similarity to MceB, the immunity protein of microcin E492. The expression of mcnI gene from a plasmid (pIN) was sufficient to confer immunity against microcin N, proving unambiguously its function as an immunity protein (data not shown). Transmembrane domain prediction of McnI and MceB using sosui (Hirokawa et al., 1998), tmpred (Hofmann Selleckchem Screening Library & Stoffel, 1993), and predictprotein (Rost et al., 2004) showed that both proteins have three

high-score transmembrane helices with the amino terminal exposed to the periplasm. Both proteins show high identity in transmembrane regions, but not in the nonhomologous regions located in the loops. This suggests similar mechanisms of immunity through an interaction with host proteins (Lagos et al., 2009) by the transmembrane regions and specific recognition of its cognate microcin by the periplasmic loops. This may explain why, despite their high similarity, microcin E492 and microcin N producers do not have cross-immunity (Sable et al., 2003). The mcnN gene encodes for the microcin N polypeptide. This polypeptide is synthesized as a prepolypeptide of 89 amino acids. The mcnN gene has three insertions with respect to the previously reported gene mtfS; these resulted in major changes in the sequence of the encoded polypeptide. The insertions in mcnN produce changes in a region of 10 amino acids located in the N-terminal domain of MtfS. This region has been involved in the toxic activity in other Gram-negative pore-forming microcins (Azpiroz

& Laviña, 2007). Processed microcin N has a deduced mass of 7221.9 Da and shares 63% of identity and 73% of similarity with the mature microcin E492. Microcin N, unlike microcin E492, lacks the C-terminal serine-rich MycoClean Mycoplasma Removal Kit region that serves as a signal for the posttranslational modification with salmochelin (Azpiroz & Laviña, 2007). Salmochelin is required for the recognition and import of microcin E492 through the catecholic receptors FepA, Fiu, and Cir (Strahsburger et al., 2005; Fischbach et al., 2006). The absence of this serine-rich region and the modifying enzymes explain the sensitivity of E. coli H1876 – a triple mutant for the catecholic receptors – to microcin N (data not shown). The production of microcin N by E. coli MC4100 pGOB18 was analyzed during the different stages of bacterial growth in Nut, LB, MH broth, and M63 (Fig. 2). It was established that E. coli MC4100 pGOB18 begins to produce microcin N during the exponential phase, when the culture has reached an OD600 nm>0.4 (approximately at 4–5 h of growth).