An important group of As(III)-oxidising bacteria belong to the Thiomonas genus, and are ubiquitous in arsenic-contaminated environments [12–15]. Thiomonas strains are able to gain energy from the oxidation of reduced inorganic sulphur compounds (RISCs) , and are defined as facultative chemolithoautotrophs
which grow optimally in mixotrophic media containing RISCs and organic supplements. These bacteria are AR-13324 nmr also capable of organotrophic growth . The original description comprised Thiomonas cuprina, T. intermedia, T. perometabolis and T. Thermosulfata [17, 18]. Thiomonas perometabolis was isolated from soil at a building site in Los Angeles, U.S.A., as Thiobacillus perometabolis . It was differentiated from Thiobacillus intermedius (now T. intermedia, the type species of the genus) as it was apparently unable to grow autotrophically. However, Katayama-Fujimura and Kuraishi  have since suggested that this is not true. Recently described species include Thiomonas. arsenivorans  and the Thiomonas strains 3As , Ynys1  and WJ68 . Thiomonas sp. 3As was obtained from the
Carnoulès mine tailings, Southern France . It was shown that this JIB04 nmr bacterium could gain energy from the oxidation of arsenic. The presence of carboxysomes and the detection of the cbbSL genes encoding ribulose 1,5-bisphosphate carboxylase/oxygenase, led the authors propose that this strain may be able to fix CO2. T. arsenivorans was isolated from another arsenic-rich mine residue at the Cheni former gold mine, Limousin, France . The Cheni site is not very acidic (pH ~6.0), but is highly contaminated with arsenic (6.0 mg g-1 in the solid phase and ~1.33 mM in the liquid phase) . T. arsenivorans has been shown to oxidise arsenic and ferrous iron, and is able to grow autotrophically
using arsenic as the sole energy BTK activity source . Strain Ynys1 was isolated from ferruginous waters which have been draining from an adit since the closure of several coal mines near to the village of Ynysarwed, Wales, U.K. . The waters were of relatively neutral pH (pH 6.3) with elevated iron loading (300 mg L-1) and have led to significant pollution of the area . Strain WJ68 was the dominant isolate obtained from effluent draining all three of the compost bioreactors of a pilot-scale bioremediation plant receiving water from the Wheal Jane Tau-protein kinase tin mine, Cornwall, U.K. . Both WJ68 and Ynys1 are known to oxidise ferrous iron, while WJ68 has been shown to oxidise arsenite . These five strains are interesting in terms of arsenic metabolism: T. arsenivorans, WJ68 and 3As are able to oxidise As(III), while Ynys1 and T. perometabolis are not. Moreover, T. arsenivorans and 3As present interesting physiological traits, in particular that these strains are able to use As(III) as an electron donor. However, differences between Thiomonas strains in the way they have adapted and respond to arsenic have never been studied further.