Finally, genes encoding for peptidyl prolyl cis trans isom erase proteins belonging to the immunophilin superfamily were found to be accumulated. PPIase proteins have general functions in protein folding and protein degradation, and several proteins Inhibitors,Modulators,Libraries have shown antifungal properties, similar to PR genes. In the down regulated genes three GO terms were noticeable with regard to the pathogen presence, while associations to the disease were rather only present after pathogen attack, after both treatments, and only after control treatment. The genes only present in response to FHB were categorised as FHB re sponsive genes. Especially, up regulated transcripts are likely to represent defences, Inhibitors,Modulators,Libraries such as trigger mechanisms or direct antimicrobial activities.
Genes with similar ex pression profiles after both treatments were categorised as genotype specific genes because they were differen tially Carfilzomib expressed to lower levels or absent in the cv. Lynx spike samples. Up regulated genes were hereafter dis cussed as members of a basal defence if their induction has been demonstrated in previous related resistance studies. Finally, a comparison of the genes differentially expressed in cv. Dream at 32 and 72 h after Fusarium inoculation was performed to separate expression changes which have been maintained from those that were exclusive for one of the two timepoints. Genes that are only differentially expressed at 72 hai were categorised as 72 hai specific genes. A mapping into one of the two categories FHB responsive genes and genotype Inhibitors,Modulators,Libraries specific genes could not be done, due to the low quality of the microarrays obtained from mock treated cv.
Lynx samples at this timepoint. The revised dataset contained a total of 2,169 differ entially expressed genes after the transcripts Inhibitors,Modulators,Libraries likely to represent general cellular processes in cv. Dream were removed. In total 374 of those genes could be assigned to 11 different defence related classes. The gene classes and assignments were made based on the GSEA results and on information obtained from FHB related literature. Table 4 gives an overview of the respective number of individual genes which have been assigned to the three categories and the 11 defence related gene classes. Detailed information on genes that were iden tified as putative defence related is provided in Additional files 1, 2 and 3. The GSEA results were contributing to the formation of the functional classes, jasmonic acid and ethylene related genes, cysteine rich anti microbial peptides including serine protease inhi bitors, jasmonate regulated proteins comprising a set of strictly FHB inducible genes, GDSL lipases, and proteolysis including serine proteases.