3 ± 2.1% during exponential phase to 66.6 ± 10.4% during stationary phase (Ipatasertib datasheet Figure 4, D3). sOUR values were not significantly different (α = 0.05) in the presence or absence of added NO2 –N/L (Figure 4, D2, Figure 2, B2,
respectively). Exponential phase relative mRNA concentrations of amoA and hao were statistically lower during growth in the presence of 280 mg NO2 –N/L than in the absence of added nitrite (Figure 4, D4, Table 4). However, exponential phase transcription of nirK and norB was significantly higher in the presence of 280 mg NO2 –N/L than in the absence of added nitrite (Figure 4, D4 and Figure 3, B4, Table 4). During stationary phase, amoA, hao, nirK and norB relative mRNA concentrations were all statistically lower in the presence of 280 mg NO2 –N/L than in the absence of added nitrite (Figure 3, B4 and Figure 4, D4, Table 4). Figure 4 Profiles BB-94 of NH 3 -N, NO 2 – -N, and NH 2 OH-N (D1), cell density and sOUR (D2), NO and fraction of NO containing cells (D3) and gene expression (D4) during exponential phase and stationary phase at DO = 1.5 mg/L in the presence of added 280 mg NO 2 – -N/L. Table 4 Statistical comparison of relative mRNA
concentrations Necrostatin-1 chemical structure and sOUR in exponential (E) and stationary (S) phase cultures grown in the presence and absence of nitrite (p values < 5.0 × 10-2 indicate statistically significant differences). Growth phase p = amoA hao nirK norB sOUR E 7.9 × 10-4 Thiamet G 1.2 × 10-3 1.3 × 10 -3 2.8 × 10 -3 7.0 × 10-3 S 5.1 × 10-5 3.2 × 10-5 3.2 × 10-5 4.6 × 10-5 2.0 × 10 -1 Underlined text indicates statistically similar results, bold text indicates statistical increase and regular text indicates decrease. Discussion Functional gene transcription and N profiles during batch growth of N. europaea In addition to its well-studied NH3 oxidation pathway, the genome of N. europaea contains genes coding for several denitrification
steps, including NO2 – and NO reduction . While significant work exists on expression analysis of amoA and to an extent, hao, [17–22], quantitative transcription patterns for nirK and norB are relatively less characterized. The significance of this study therefore lies in elucidating the co-transcription patterns of amoA, hao, nirK and norB under varying degree of DO and NO2 – exposure during batch growth of N. europaea. The general overall reduction in amoA transcription during the stationary phase, at DO = 0.5 and 1,5 mg O2/L (Figure 3, A4-B4), can be linked to dwindling energy resources for N. europaea [15, 23] or toxicity of accumulating NO2 – concentrations . The higher amoA relative mRNA concentrations during the stationary phase at DO = 3.0 mg O2/L were not expected and likely due to the opposing trends in exponential phase and stationary phase responses to increasing DO concentrations (Figure 3, B4-D4), as discussed below.